Choosing an HPLC Column Supplier

 

When choosing an HPLC column supplier, you need to consider your operational conditions and the specifications of the column. There is a risk of using the column outside its specification, so it is crucial to monitor its performance. The following are a few tips to avoid problems with your column: Read the specification sheet carefully before using it, and follow the recommended operating conditions.Click here; www.chromtech.com for more information about chromatography supplies and equipment.

 

Consider the sample throughput of the HPLC column. The higher the sample throughput, the more plates the column can handle. It can also accommodate higher-pH and pressures. You may also need to consider the lifetime of your column, so use the maximum flow rate recommended by the manufacturer. It is a good idea to monitor the column's health and make adjustments as needed.

 

Choose an appropriate particle size for your HPLC column. Larger particles give better resolution, while smaller ones give you better sensitivity and less run time. Particle size also affects the amount of backpressure. For example, a 15-cm column operating at 11 Mpa backpressure has a good resolution.At www.chromtech.com you will get a variety of column sizes that meet your application needs.

Lastly, make sure the column is able to handle organic solvents. These may require slower ramping. You can also use a low flow rate to make changes more gradual. This will extend the life of your column. Your column supplier should also be willing to help you monitor and maintain the quality of your column.

 

HPLC columns are a crucial component in HPLC applications. These columns help you to separate sample components. As the mobile phase passes through the column, the sample will separate into its components. Generally, HPLC columns are filled with silica gel. Its size and porosity make it the ideal choice. Silica gel is inert, so it will not react with the mobile phase and will not interfere with the analysis. It's also called the stationary phase.

 

To select an HPLC column, make sure to read the specifications carefully. If you want to use an additive, make sure the column you choose is compatible with the compounds you want to use. Some additives may alter the performance of the column, and this can cause a problem. It's better to use a dedicated SEC column if you're not sure.

 

There are a number of types of stationary phases available on the market. They vary in their chemical structures and packing styles. Some have special features that allow them to perform specific applications more effectively. Generally, though, a good choice is a combination of several advantages. For example, a monolithic column is much more durable than a particulate one.For more info,please visit:https://en.wikipedia.org/wiki/Paper_chromatography